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Hair Sample Test: Definition, How It Works, and the Factors Affecting the Test Results

26 February, 2024

A lab technician holding hair samples with a tweezer and petri dish

A hair sample test is a type of drug test that analyses metabolites extracted from hair strands. It works by chemically extracting metabolites from a small sample of hair. This type of test can provide a longer detection window compared to other methods like urine or blood tests. However, various factors can affect the test results. These include detection windows, individual factors, and cut-off levels set by the testing facility.

Identifying substance abuse is important in various settings. Individuals with drug or alcohol addiction have a higher likelihood of experiencing poisoning and developing long-term health conditions. Additionally, it poses risks to road and workplace safety. Therefore, drug testing becomes crucial in maintaining a safe and healthy community. One of the methods is hair testing. This article will provide an overview of hair testing, including its mechanism of action and factors affecting test results.

What is a Hair Sample Test?

A hair sample test is a non-invasive type of drug testing that can detect a wide range of substances. It involves collecting a small sample of hair, usually from the scalp. People can use their body hair if head hair is not available. The collected sample is secured and sent to a laboratory for analysis.

The results from the hair test can provide information about the type of drug present, the frequency of usage, and the approximate timeline of use. Moreover, it is helpful in various settings, such as employment screening, legal proceedings, and substance abuse treatment programs. This can help employers, courts, and health professionals if a person has been using illegal drugs or alcohol.

Furthermore, hair drug testing is a relatively new forensic or legal testing method. With recent developments in technology, it has emerged as a valuable tool for determining substance use accurately. However, this test can be relatively expensive due to its complex process and has a longer turnaround time for results.

Advantages Over the Other Testing Methods

  • A hair drug test has a longer detection period than saliva, blood, and urine tests.
  • The sample collection process is non-invasive and painless.
  • It can provide a cumulative history of drug use from several months before testing.
  • It is difficult to cheat or tamper with the results. Hence, it ensures a higher level of accuracy and reliability.
  • The human hair structure remains stable, making it difficult for the metabolites to be washed out or diluted.
  • External contaminants like dirt and sweat do not affect the hair sample.
  • It can provide evidence of drug use in cases where other methods might yield false negatives.

Three professionals conducting a laboratory test

How a Hair Sample Test Works

A hair sample test analyses the sample for the presence of specific metabolites that are present in the hair shaft. When a person consumes drugs or alcohol, the liver breaks them down into various metabolites. These metabolites then enter the bloodstream and are eventually deposited into the hair follicles. Traces of these substances are detectable as hair grows.

The process begins with the collection of a sample of hair from the individual. This sample is usually from the back of the head, close to the scalp, to minimise the impact of any hair treatments. Subsequently, the sample is then sent to a laboratory for hair evaluation. In the facility, the conductor will wash the hair to remove any external contaminants.

Then, the remaining hair undergoes chemical processes to extract any drug or alcohol metabolites. Furthermore, the conductor will analyse the compounds using highly sensitive techniques to identify and quantify the substance of interest. The results of the hair follicle drug tests typically show the presence or absence of substances.

Techniques to Analyse Hair Samples

Analysing hair samples involves using different techniques. One method is the immunoassay test, which experts commonly employ for initial screenings. This test relies on immunoassays or antibodies to spot drugs of abuse and their byproducts in the hair sample. It gives a simple yes-or-no result, showing whether drugs are present or not.

Another technique is Gas Chromatography-Mass Spectrometry (GC-MS), which is the gold standard for this type of analysis. GC-MS is primarily used for confirmatory tests and can accurately quantify the substances. This method can provide detailed information about the types and amounts of drugs or other substances in the hair sample.

A lab technician inserting hair samples in a test tube using a tweezer

Factors Affecting Hair Sample Test Results

There are several factors that can affect hair sample test results. One important factor is the detection window. It denotes the period during which drugs or their metabolites remain detectable in the hair. The detection of drug use for hair analysis is typically longer than that of other types of drug tests.

In addition, individual factors can impact hair testing results. These include the body weight, tolerance, metabolism, gender, and age. These factors can affect the concentration of drugs in the hair. For instance, heavier people might show lower drug concentrations in hair compared to lighter individuals.

Moreover, cut-off levels are another important factor. These are the minimum concentrations of drugs that must be present in the hair for the test to be considered positive. Different drug classes may have different cut-off levels. It is important to consider these factors when interpreting hair test results to avoid false positives. It also determines if follow-up tests or confirmatory testing is necessary.

External Contaminant Factors

External factors such as hair treatments, including hair dyes, can significantly affect the accuracy of hair strand test results. These treatments can alter the composition of the hair, making it difficult to detect the presence of illicit drugs. Additionally, some cosmetic treatments like bleaching, dyeing, or perming can interfere with the detection process.

Furthermore, medications and personal care products such as shampoos, conditioners, and hair styling products may cross-react with the drugs being tested. This can result in false positives, where the test incorrectly indicates the presence of drugs. Overall, these factors are some important considerations that need to be taken into account.

Conclusion

A hair sample test is a non-invasive method that can detect drugs or alcohol in the system of a person. It analyses hair for specific metabolites left in hair follicles after substance use. After collecting the sample, the officer will send it to a lab. The lab washes and processes the hair to extract any substance traces. Results show if drugs or alcohol were present. This method can be costly and time-consuming.

Furthermore, analysing hair samples involves various techniques like immunoassay and GC-MS, with the latter being the gold standard for accuracy. Factors affecting test results include the detection window, individual traits like body weight and metabolism, and cut-off levels. External contaminants, such as hair treatments and certain products, can also impact accuracy, potentially leading to false positives. Understanding these factors is crucial for interpreting results accurately and determining the need for further testing.

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